Johnson M H, Thompson E J
J Clin Pathol. 1982 Dec;35(12):1328-33. doi: 10.1136/jcp.35.12.1328.
A development of the disc polyacrylamide gel electrophoretic system of Ornstein and Davis, which has been applied to the analysis of unconcentrated cerebrospinal fluid, is described. Modifications to the scanning densitometer have improved the signal:noise ratio of the gel scanning system. Using this technique, we have studied the dye-binding properties of albumin, and of beta- and gamma-globulins, and have shown that reproducible quantification of proteins can be achieved. The advantages of electrophoretic methods over immunochemical methods of quantification are discussed.
本文描述了一种对奥恩斯坦和戴维斯圆盘聚丙烯酰胺凝胶电泳系统的改进，该系统已应用于未浓缩脑脊液的分析。对扫描密度计的改进提高了凝胶扫描系统的信噪比。利用这项技术，我们研究了白蛋白、β球蛋白和γ球蛋白的染料结合特性，并表明可以实现蛋白质的可重复定量。文中还讨论了电泳定量方法相对于免疫化学定量方法的优势。
