Fredrikson G, Krabisch L, Belfrage P
J Lipid Res. 1982 Nov;23(8):1246-8.
Procedures for the synthesis and purification of two 14C-labeled alkyl polyoxyethylene detergent preparations are described. One of the preparations consisted mainly of [14C]dodecyloctaoxyethylene ether, the other consisted mainly of [14C] octylhexaoxyethylene ether, with critical micellar concentrations of approximately 50 microM and 10 mM, respectively. Condensation of alkyl bromides with fractionated polyethyleneglycol resulted in less polydisperse products than conventional ethylene oxide condensation. Highly purified detergent species could easily be obtained from the preparations by thin-layer chromatography. Their use as tracers greatly simplifies determination of detergent concentration during protein solubilization and fractionation.
描述了两种14C标记的烷基聚氧乙烯洗涤剂制剂的合成和纯化程序。其中一种制剂主要由[14C]十二烷基八氧乙烯醚组成，另一种主要由[14C]辛基六氧乙烯醚组成，其临界胶束浓度分别约为50微摩尔/升和10毫摩尔/升。与传统的环氧乙烷缩合相比，烷基溴与分馏聚乙二醇的缩合产生的产物多分散性较小。通过薄层色谱法可以很容易地从制剂中获得高度纯化的洗涤剂种类。它们作为示踪剂的使用极大地简化了蛋白质溶解和分级分离过程中洗涤剂浓度的测定。
