Cornford E M, Huot M E, Diep C P, Rowley G A
J Parasitol. 1982 Dec;68(6):1010-20.
The volume of distribution (= VD) of water was measured in Schistosoma mansoni, S. haematobium, S. japonicum, and Hymenolepis diminuta. In the rat tapeworm, H. diminuta, the volume of distribution of 3H-water was positively correlated with wet weight (r = 0.87, P less than 0.001) and this same phenomenon also was demonstrated in S. mansoni (r = 0.90, P less than 0.001). The 5-sec VDwater was constant over a range of glucose (0.01-50 mM) and phenylalanine (0.01-20 mM) concentrations in both male and female schistosomes. The VD of antipyrine, which permeates by virtue of its lipophilic properties, also was shown to correlate with protein content in S. mansoni. Protein content determined in single, isolated schistosomes was correlated with the volume of distribution of water in S. haematobium, S. japonicum, and S. mansoni males and females. Age-related variations in the protein content (of S. mansoni) and volume of distribution of water (in both S. japonicum and S. mansoni) were also defined, and the use of tritiated water content as an indicator of mass in small tissue samples was thus established. Female blood flukes recovered from mice infected for more than 90 days appeared to be characterized by a slight reduction in size with age. Schistosoma mansoni reared in the golden hamster may be slightly smaller than schistosomes of the same strain raised in outbred mice. These results provide a baseline that should be useful for future physiological and immunological studies.
在曼氏血吸虫、埃及血吸虫、日本血吸虫和微小膜壳绦虫中测量了水的分布容积（= VD）。在大鼠绦虫微小膜壳绦虫中，3H-水的分布容积与湿重呈正相关（r = 0.87，P < 0.001），并且在曼氏血吸虫中也证实了同样的现象（r = 0.90，P < 0.001）。在雄性和雌性血吸虫中，5秒VD水在一系列葡萄糖（0.01 - 50 mM）和苯丙氨酸（0.01 - 20 mM）浓度范围内保持恒定。凭借其亲脂性渗透的安替比林的VD也显示与曼氏血吸虫中的蛋白质含量相关。在单个分离的血吸虫中测定的蛋白质含量与埃及血吸虫、日本血吸虫以及曼氏血吸虫雌雄个体中的水分布容积相关。还确定了（曼氏血吸虫的）蛋白质含量和（日本血吸虫和曼氏血吸虫中的）水分布容积的年龄相关变化，从而确立了使用氚化水含量作为小组织样本质量指标的方法。从感染超过90天的小鼠中回收的雌性血吸虫似乎随着年龄增长尺寸略有减小。在金黄仓鼠中饲养的曼氏血吸虫可能比在远交系小鼠中饲养的同一品系的血吸虫略小。这些结果提供了一个基线，应该对未来的生理学和免疫学研究有用。
