Gilboa E, Kolbe M, Noonan K, Kucherlapati R
J Virol. 1982 Dec;44(3):845-51. doi: 10.1128/JVI.44.3.845-851.1982.
A 0.9-kilobase DNA fragment from the genome of Moloney murine leukemia virus, including the viral long terminal repeat, was covalently linked to the herpes simplex virus I thymidine kinase (tk) gene whose promoter was previously removed. The hybrid DNA structure was introduced into the chromosome of tk- mouse cells at single copy numbers, via transfection procedures. Cells expressing the newly introduced tk gene were identified by the HAT selection procedure and analyzed for tk- and moloney murine leukemia virus-specific DNA and RNA sequences by blot hybridization procedures. Expression of the tk gene is dependent on function(s) provided in cis by the viral DNA fragment. Vectors derived from this region are termed rGag (rG) vectors.
来自莫洛尼鼠白血病病毒基因组的一个0.9千碱基对的DNA片段，包括病毒长末端重复序列，与单纯疱疹病毒I胸苷激酶（tk）基因共价连接，该基因的启动子先前已被去除。通过转染程序，将这种杂交DNA结构以单拷贝数引入tk-小鼠细胞的染色体中。通过HAT选择程序鉴定表达新引入的tk基因的细胞，并通过印迹杂交程序分析tk-和莫洛尼鼠白血病病毒特异性的DNA和RNA序列。tk基因的表达依赖于病毒DNA片段顺式提供的功能。源自该区域的载体被称为rGag（rG）载体。
