Vovk A I, Parkhomenko Iu M, Babicheva A F, Chernysh I Iu
Ukr Biokhim Zh (1978). 1982 Nov-Dec;54(6):674-8.
A modification of the method for determining the content of acetoin in biological media is described; the method is based on formation of a stained complex under acyloin heating with the acidified ammonium molybdate water solution. Colorimetric determination of the acetoin amount in the enzymic system containing sodium pyruvate, thiamine pyrophosphate and tris-HCl is shown to be possible. Application of the method to analysis of the catalytic activity of pyruvate dehydrogenase preparations from different sources and with different degree of purification in the reaction of acetoin formation from pyruvate is described.
描述了一种用于测定生物介质中乙偶姻含量的方法的改进；该方法基于在乙偶姻与酸化钼酸铵水溶液加热时形成染色络合物。结果表明，比色法可用于测定含有丙酮酸钠、硫胺素焦磷酸和三羟甲基氨基甲烷盐酸盐的酶系统中乙偶姻的含量。描述了该方法在分析来自不同来源且具有不同纯化程度的丙酮酸脱氢酶制剂在由丙酮酸形成乙偶姻反应中的催化活性方面的应用。
