Hirvonen J, Penttinen J, Huttunen P
Z Rechtsmed. 1982;89(2):81-8. doi: 10.1007/BF02092373.
Vital reactions in the paw skin of guinea pigs dying of hypothermia were examined using a variety of histochemical methods to find ways of distinguishing between ante-mortem and post-mortem frostbites. Some animals were treated with ethanol or diazepam. Edema, hyperemia, and incipient accumulation of granulocytes were seen to develop within the 2-6 h for which the animals had survived in the cold (-20 degrees C). The granulocytes were first seen in the capillaries beneath the epithelial papillae and thereafter deeper in the dermis. They mostly accumulated inside the venules in the endothelium, but there were cases in which emigration had started. The best method for demonstrating the reaction appeared to be the alkaline phosphatase reaction, which is strong in guinea pig granulocytes and highlighted the latter well against a negative background. The conclusion is that it is possible to detect a positive vital reaction, but methodologic investigations are needed before similar changes can be visualized in cases of acute hypothermia in human beings.
利用多种组织化学方法检查死于体温过低的豚鼠爪部皮肤的生命反应，以寻找区分生前冻伤和死后冻伤的方法。一些动物用乙醇或地西泮进行处理。在动物于寒冷环境（-20摄氏度）中存活的2至6小时内，可见水肿、充血以及粒细胞的初期积聚。粒细胞首先出现在上皮乳头下方的毛细血管中，随后出现在真皮更深层。它们大多在内皮小静脉内积聚，但也有开始游出的情况。显示这种反应的最佳方法似乎是碱性磷酸酶反应，该反应在豚鼠粒细胞中较强，能在阴性背景下很好地凸显粒细胞。结论是有可能检测到阳性生命反应，但在能够在人类急性体温过低病例中观察到类似变化之前，还需要进行方法学研究。
