Ebner H, Gebhart W, Lassmann H, Jurecka W
Acta Derm Venereol. 1977;57(2):133-6.
The proliferative activity of epidermal structures was determined in 6 patients suffering from lichen planus (i.p.). By using the in vitro 3H-thymidine labelling technique, semithin Epon sections of lesional and perilesional uninvolved skin were compared autoradiographically with normal controls. The following results were obtained. The labelling index of epidermal cells was significantly increased within the i.p. papules. The mean values ranged from 41% in acanthotic i.p. to 23% in atrophic i.p. whereas in adjacent unaffected skin (15% and 11% respectively) as well as in normal controls (6%) a lower 3H-index was observed. As regards damage to basal cells within i.p. lesions, a factor--the relation between the number of basal cells in involved and uninvolved skin in reference to the epidermal length--was calculated in order to correct the autoradiographic results in involved skin. The differences between i.p. papules, adjacent skin and controls remained statistically significant even after correction. The intradermal eccrine ducts revealed a significantly increased labelling index in i.p. lesions as compared with perilesional and normal skin. In this respect no difference could be observed between the acanthotic and the atrophic variant of i.p. These findings indicate that replacement of damaged basal cells in i.p. is achieved by an increase in actively dividing keratinocytes in both epidermis and skin appendages within the lesion.
采用体外3H-胸腺嘧啶核苷标记技术，对6例扁平苔藓患者（皮损内注射）的表皮结构增殖活性进行了测定。将皮损及皮损周围未受累皮肤的半薄Epon切片与正常对照进行放射自显影比较。结果如下。皮损内丘疹表皮细胞的标记指数显著升高。棘层肥厚性扁平苔藓的平均值为41%，萎缩性扁平苔藓为23%，而相邻未受累皮肤（分别为15%和11%）以及正常对照（6%）的3H指数较低。关于皮损内基底细胞的损伤，计算了一个因子——受累皮肤和未受累皮肤中基底细胞数量与表皮长度的关系，以校正受累皮肤的放射自显影结果。即使校正后，扁平苔藓丘疹、相邻皮肤和对照之间的差异仍具有统计学意义。与皮损周围和正常皮肤相比，皮损内真皮内小汗腺导管的标记指数显著升高。在这方面，扁平苔藓的棘层肥厚型和萎缩型之间未观察到差异。这些发现表明，扁平苔藓中受损基底细胞的替代是通过皮损内表皮和皮肤附属器中活跃分裂的角质形成细胞增加来实现的。
