Campbell T M, Studdert M J
Am J Vet Res. 1982 Nov;43(11):1922-5.
An inactivated equine adenovirus type 1 (EAdV1) vaccine was administered to 4 horses. The horses had virus-neutralizing (VN) antibody titers before they were vaccinated, but developed higher VN antibody titers in response to vaccination. Nonvaccinated control horses did not show increases in VN antibody during the study, indicating that any increase in antibody titer in vaccinated horses was a result of vaccination and not due to an EAdV1 epizootic during the study. Specific EAdV1 in vitro lymphocyte blastogenesis (LB) was evaluated, using lymphocytes from 4 vaccinated and 2 control horses. Horses were vaccinated on days 0 and 14, and the LB assays were conducted on days -4, 0, 3, 7, 10, 14, 17, 21, 24, and 28. Lymphocytes from horses were incubated for 4 days with 2 concentrations of inactivated, concentrated and purified EAdV1 antigen. The LB responses for the 2 control horses showed no significant changes during the study period (maximum stimulation indices to EAdV1 antigen for individual horses were between 2.8 and 3.6). The 4 vaccinated horses showed marked increases in stimulation indices in response to EAdV1 antigen (maximum stimulation indices, between 5.3 and 18.6). In control assays, identical lymphocyte preparations from all horses responded normally to phytohemagglutinin.
给4匹马接种了1型马腺病毒（EAdV1）灭活疫苗。这些马在接种疫苗前具有病毒中和（VN）抗体滴度，但接种疫苗后产生了更高的VN抗体滴度。未接种疫苗的对照马在研究期间VN抗体未出现升高，这表明接种疫苗的马抗体滴度的任何升高都是接种疫苗的结果，而非研究期间EAdV1 epizootic所致。使用4匹接种疫苗的马和2匹对照马的淋巴细胞评估了特定的EAdV1体外淋巴细胞增殖（LB）情况。马在第0天和第14天接种疫苗，并在第-4、0、3、7、10、14、17、21、24和28天进行LB试验。将马的淋巴细胞与2种浓度的灭活、浓缩和纯化的EAdV1抗原孵育4天。2匹对照马的LB反应在研究期间无显著变化（个体马对EAdV1抗原的最大刺激指数在2.8至3.6之间）。4匹接种疫苗的马对EAdV1抗原的刺激指数显著增加（最大刺激指数在5.3至18.6之间）。在对照试验中，所有马相同的淋巴细胞制剂对植物血凝素反应正常。
