Barsoum Y H, Pickard W F
Bioelectromagnetics. 1982;3(4):393-400. doi: 10.1002/bem.2250030402.
Single giant cells of Chara braunii and Nitella flexilis were placed in a microstrip exposure apparatus and subjected to bursts of electromagnetic radiation (carrier frequencies from 200 to 8,200 MHz) at a nominal power level of 100 W/m2. The vacuolar potential was monitored with a micropipette, and offsets as low as 1 microV could be resolved in real time by suitable filtering and signal averaging; under these conditions, no offsets of the vacuolar potential were detected. At much higher power levels (corresponding to greater than or equal to 2 V rms between microstrip and ground plane), the slow hyperpolarizing ramp reported at lower frequencies could be seen but, because of insufficient power, could not be accurately measured. It appeared to decay beyond 500 MHz and to be absent at and above 950 MHz. To investigate reports that snail neurons irradiated for 1 h at 2,450 MHz and approximately 15.5 W/kg developed lowered membrane resistivities, Characean cells were exposed in the microstrip apparatus for 1 h at 2,450 MHz and 230 W/m2; their membrane resistivities were found to be lowered about 18.5%.
将轮藻和柔膜丽藻的单个巨型细胞置于微带暴露装置中,并在标称功率水平为100 W/m²的情况下,使其受到电磁辐射脉冲(载波频率为200至8200 MHz)的照射。用微量移液器监测液泡电位,通过适当的滤波和信号平均,可实时分辨低至1微伏的偏移;在这些条件下,未检测到液泡电位的偏移。在更高的功率水平下(对应于微带和接地平面之间大于或等于2 V rms),可以看到在较低频率下报告的缓慢超极化斜坡,但由于功率不足,无法准确测量。它似乎在500 MHz以上衰减,在950 MHz及以上不存在。为了研究有关蜗牛神经元在2450 MHz和约15.5 W/kg的条件下照射1小时后膜电阻降低的报道,将轮藻类细胞在微带装置中于2450 MHz和230 W/m²的条件下暴露1小时;发现它们的膜电阻降低了约18.5%。