异体骨骺软骨细胞移植至关节面。一项在兔身上的实验研究。

Bentley G, Smith A U, Mukerjhee R

Ann Rheum Dis. 1978 Oct;37(5):449-58. doi: 10.1136/ard.37.5.449.

Isolated epiphyseal chondrocytes from 5 week old female New Zealand white rabbits were transplanted as allografts into drill holes in the tibial articular surface of adult male New Zealand white rabbits. The grafts were examined after 8 weeks. Fresh chondrocytes which were partially separated from their matrix were more successful (47%) than completely separated cells (20%) and were significantly more successful (P greater than 0.05) in skeletally mature (58%) as opposed to immature recipients (20%). Storage of the cells at -79 degrees C for 2 to 9 days or at -196 degrees C for 36 to 58 weeks gave successful results of 23% and 33%, respectively. Control defects showed fibrocartilage filling the defect in 25%. The factors affecting survival of chondrocyte allografts require further study before clinical application of the method.

从5周龄雌性新西兰白兔分离出的骺软骨细胞作为同种异体移植物，被移植到成年雄性新西兰白兔胫骨关节面的钻孔中。8周后对移植物进行检查。与基质部分分离的新鲜软骨细胞移植成功率更高（47%），高于完全分离的细胞（20%），并且在骨骼成熟的受体中（58%）比未成熟受体（20%）的成功率显著更高（P大于0.05）。细胞在-79℃储存2至9天或在-196℃储存36至58周，移植成功率分别为23%和33%。对照缺损显示25%有纤维软骨填充缺损。在该方法临床应用之前，影响软骨细胞同种异体移植存活的因素需要进一步研究。