Verdone-Smith C, Enesco H E
Exp Gerontol. 1982;17(6):463-71. doi: 10.1016/s0531-5565(82)80008-9.
Dietary restriction in the rotifer Asplanchna brightwelli, carried out by lengthening the interval between feedings, causes an increase both in mean lifespan and in the length of the reproductive period. In the present study, we found that the rate of nuclear division in the gastric glands and vitellarium of the rotifer, as determined by daily nuclear counts, was retarded by dietary restriction. Spectro-fluorimetric measurements show that total DNA content remains constant from the beginning to the end of the lifespan, and is unaffected by dietary restriction. Lactic dehydrogenase and malic dehydrogenase activities were also measured throughout the lifespan of the rotifer, and were not affected by dietary restriction.
通过延长喂食间隔对轮虫明亮镜轮虫进行饮食限制,会导致平均寿命和生殖期长度增加。在本研究中,我们发现,通过每日细胞核计数确定,轮虫胃腺和卵黄腺中的细胞核分裂速率因饮食限制而减缓。荧光分光光度法测量表明,从寿命开始到结束,总DNA含量保持恒定,且不受饮食限制的影响。在轮虫的整个寿命期内还测量了乳酸脱氢酶和苹果酸脱氢酶的活性,它们不受饮食限制的影响。