Pasargiklian I, Lusco G, Mascheroni E, Ranzi M L
Quad Sclavo Diagn. 1982 Sep;18(3):354-60.
A screening method for the rapid detection of bacteriuria through a combination of filtration and staining has been evaluated. 307 urine samples have been tested with both the regular colony enumeration on culture media and the rapid method. Taking 10(5) CFU/ml as a cut-off, the agreement was reached for 279 (90.9%) samples, 65 (21.2%) of which positive and 214 (69.7%) negative. Only 4 (1.3%) false negative and 24 (7.8%) false positive were obtained; false positive (whose real significance is further discussed) anyhow can be corrected after cultural confirmation. If the cut-off is lowered to 10(4) CFU/ml the frequency of agreements is slightly less (88.6%). From the operational side, the method is unsensitive to the antimicrobial content of the urines, and appears easy to apply and quite rapid: a sample needs not longer than 2 minutes to be processed.
一种通过过滤和染色相结合的快速检测菌尿症的筛查方法已得到评估。307份尿液样本同时采用常规培养基菌落计数法和快速检测法进行了检测。以10⁵CFU/ml作为临界值，279份（90.9%）样本结果一致，其中65份（21.2%）为阳性，214份（69.7%）为阴性。仅得到4份（1.3%）假阴性和24份（7.8%）假阳性结果；假阳性结果（其实际意义将进一步讨论）无论如何都可在培养确认后得到纠正。如果将临界值降至10⁴CFU/ml，结果一致的频率略低（88.6%）。从操作方面来看，该方法对尿液中的抗菌成分不敏感，且似乎易于应用且相当快速：一份样本处理时间不超过2分钟。
