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[Multistat III离心机分析仪在改进的天冬氨酸氨基转移酶和丙氨酸氨基转移酶活性测定方法中的实际应用]

[Practical use of the Multistat III centrifuge analyzer in an improved method for the determination of the activity of aspartate aminotransferase and alanine aminotransferase].

作者信息

Ferrari G, Erba P N

出版信息

Quad Sclavo Diagn. 1982 Sep;18(3):368-76.

PMID:7184050
Abstract

We applied three procedures for measuring aspartate aminotransferase and alanine aminotransferase activities to the Multistat III MCA. Assay conditions were almost exactly as prescribed by the Scandinavian Committee on Enzymes and the International Federation of Clinical Chemistry. Owing to the overall good performance of the analyzer and its loader module, results of 20 samples will be available in 10 minutes, including preincubation with pyridoxal phosphate. Details of procedures are given; there are no differences in activities of both aminotransferases determined with three methods, allowing the user to take a choice according to personal preference.

摘要

我们对Multistat III MCA应用了三种测定天冬氨酸转氨酶和丙氨酸转氨酶活性的方法。分析条件几乎完全按照斯堪的纳维亚酶委员会和国际临床化学联合会的规定。由于分析仪及其加载模块的整体性能良好,20个样本的结果在10分钟内即可获得,包括与磷酸吡哆醛的预孵育。文中给出了方法的详细信息;用三种方法测定的两种转氨酶的活性没有差异,用户可根据个人喜好进行选择。

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