Ezaki K, Okabe K, Domyo M, Abe K, Ogawa M
Gan To Kagaku Ryoho. 1982 Feb;9(2):238-43.
Several immunological studies were performed in patients with various malignant diseases under treatment of human fibroblast interferon (HFIF). Lymphocyte natural killer (NK) activity against culture cell lines was measured before and at various time after HFIF treatment. The majority of patients reached the highest level of NK activity at 18-24 hours, mostly at 24 hours after initiating HFIF. In one group of patients, thereafter, NK activity remained the highest level during HFIF treatment, and in another group of patients, NK activity declined even with daily infusion of HFIF but usually remained above pre-treatment level. There seemed to be no correlation between NK activity and clinical activity. In contrast to NK activity against culture cell lines, an increase in lymphocyte cytotoxic activity against autologous tumor cells was not observed following HFIF treatment. Mixed lymphocyte tumor cell reaction performed in 6 patients showed that a slight increase of 3H-thymidine uptake was seen in one patient, but the rest of them had no change. In vitro sensitization to assess the in vitro generation of cytotoxic cells were negative in all 6 patients. Lymphocyte blastogenic responses to non-specific mitogens showed no significant change. Delayed type hypersensitivity reaction to recall antigens was increased in about half of the patients after HFIF treatment.
对接受人成纤维细胞干扰素（HFIF）治疗的各类恶性疾病患者进行了多项免疫学研究。在HFIF治疗前及治疗后的不同时间测量了淋巴细胞对培养细胞系的自然杀伤（NK）活性。大多数患者在18 - 24小时达到NK活性的最高水平，多数在开始HFIF治疗后24小时。此后，在一组患者中，NK活性在HFIF治疗期间维持在最高水平，而在另一组患者中，即使每日输注HFIF，NK活性仍下降，但通常维持在治疗前水平之上。NK活性与临床活性之间似乎没有相关性。与对培养细胞系的NK活性不同，HFIF治疗后未观察到淋巴细胞对自体肿瘤细胞的细胞毒性活性增加。对6例患者进行的混合淋巴细胞肿瘤细胞反应显示，1例患者的3H - 胸腺嘧啶核苷摄取略有增加，但其余患者无变化。评估细胞毒性细胞体外生成的体外致敏在所有6例患者中均为阴性。淋巴细胞对非特异性有丝分裂原的增殖反应无显著变化。HFIF治疗后约一半患者对回忆抗原的迟发型超敏反应增强。
