Kitano M, Nagase T
Gan To Kagaku Ryoho. 1982 Apr;9(4):590-8.
The sensitivity of anti-cancer drugs against cultured human lung cancer cells and the first cultured xenograft tumor has been measured by the microcolonies inhibition test in microplates (Falcon, Micro Test II). The results obtained were as follows; 1). The drug sensitivity of cancer cells has differed in each case. The established cancer cell lines of the same cell type and the same growth speed have also showed a different sensitivity. Therefore, it suggests that the measurement of drug sensitivity of cultured human cancer cells is usefull for the clinical application. 2). The drug sensitivity of cancer cells has been elevated when they changed their character during the passage cultures. On the other hand, the successful culture of cancer cells has been elevated when they were purified with discontinuous density gradients and a hypotonization. 3). In future, further efforts to develop a better medium for the colonies inhibition test, i. e., a conditioned medium containing the specific growth factor for the colony of cancer cells are imperative.
已通过微孔板（Falcon，微量检测II型）中的微集落抑制试验，测定了抗癌药物对培养的人肺癌细胞和首个培养的异种移植肿瘤的敏感性。获得的结果如下：1）。癌细胞的药物敏感性在每种情况下都有所不同。相同细胞类型和相同生长速度的已建立癌细胞系也表现出不同的敏感性。因此，这表明测定培养的人癌细胞的药物敏感性对临床应用有用。2）。癌细胞在传代培养过程中改变其特性时，其药物敏感性会提高。另一方面，当癌细胞通过不连续密度梯度和低渗处理进行纯化时，其成功培养率会提高。3）。未来，必须进一步努力开发用于集落抑制试验的更好培养基，即含有癌细胞集落特异性生长因子的条件培养基。
