Falkenstein D, Conti G T, Ajzen H, Draibe S A, Figueiredo J F
Braz J Med Biol Res. 1982 Dec;15(6):361-5.
The effect of storage of cortical slices in modified Collins solution on the viability of rabbit proximal convoluted tubules (PCT) and pars recta (PR) was evaluated by microperfusion of isolated tubules in vitro. Cortical slices of rabbit kidney were stored for 1, 24, 48, 72 and 97 h at 4 degrees C in modified Collins solution. PCT and PR were dissected from these slices and perfused with a modified Krebs-Henseleit solution in vitro. Fluid absorption (JV) was 1.21 +/- 0.3 nl min-1 mm-1 (N = 8) and the transepithelial potential difference (PD) was -2.3 +/- 0.3 mV (N = 6) for PCT from cortical slices stored 0 to 3 h. JV was 1.01 +/- 0.09 nl min-1 mm-1 (N = 8) and PD was -1.9 +/- 0.6 mV (N = 5) for PCT from tissue stored 23 to 97 h. JV of PR from tissue stored 5 to 26 h was 0.75 +/- 0.1 nl min-1 mm-1 (N = 5) and PD was -1.5 +/- 0.3 mV (N = 5). JV was 0.76 +/- 0.1 nl min-1 mm-1 (N = 3) for PR stored 72 to 74 h, and PD was not measured. There was no correlation between storage time and JV or PD. Furthermore, the JV and PD values for these tubules were similar to those obtained for freshly dissected tubules. These results show that the isolated tubule preparation is useful for the direct assessment of the efficacy of different kidney preservation solutions on nephron function.
通过体外对分离的肾小管进行微量灌注，评估了改良柯林斯溶液中皮质切片的储存对兔近端曲管（PCT）和直部（PR）活力的影响。兔肾皮质切片在4℃下于改良柯林斯溶液中储存1、24、48、72和97小时。从这些切片中分离出PCT和PR，并在体外用改良的克雷布斯 - 亨泽莱特溶液进行灌注。储存0至3小时的皮质切片中PCT的液体吸收（JV）为1.21±0.3 nl min-1 mm-1（N = 8），跨上皮电位差（PD）为-2.3±0.3 mV（N = 6）。储存23至97小时的组织中PCT的JV为1.01±0.09 nl min-1 mm-1（N = 8），PD为-1.9±0.6 mV（N = 5）。储存5至26小时的组织中PR的JV为0.75±0.1 nl min-1 mm-1（N = 5），PD为-1.5±0.3 mV（N = 5）。储存72至74小时的PR的JV为0.76±0.1 nl min-1 mm-1（N = 3），未测量PD。储存时间与JV或PD之间无相关性。此外，这些肾小管的JV和PD值与新鲜分离的肾小管相似。这些结果表明，分离的肾小管制剂可用于直接评估不同肾脏保存溶液对肾单位功能的疗效。
