鱿鱼晶状体细胞骨架和膜蛋白。
Squid lens cytoskeleton and membrane proteins.
作者信息
Siezen R J
出版信息
Curr Eye Res. 1982;2(9):579-89. doi: 10.3109/02713688208996358.
Cytoskeletal and membrane proteins were isolated from respectively the urea-soluble and urea-insoluble fractions of the squid lens. The main cytoskeletal polypeptide has a molecular weight (63000) and an amino acid composition similar to those of vertebrate intermediate filament proteins, including mammalian lens vimentins. Intermediate filaments, and bundles thereof, were regenerated from the squid lens urea-soluble fraction upon removal of urea. The main membrane polypeptide of 140000 Mr has an amino acid composition entirely different from that of the main intrinsic membrane protein of 26000 Mr which is found in all vertebrates. Although non-EDTA-extractable, the 140000 Mr squid membrane polypeptide is best classified as extrinsic, since it has a high polarity (mainly acidic residues) and can be degraded completely upon trypsin treatment of squid lens membranes.
从鱿鱼晶状体的尿素可溶部分和尿素不溶部分分别分离出细胞骨架蛋白和膜蛋白。主要的细胞骨架多肽分子量为63000，其氨基酸组成与脊椎动物中间丝蛋白相似，包括哺乳动物晶状体波形蛋白。去除尿素后，从鱿鱼晶状体尿素可溶部分再生出中间丝及其束状结构。分子量为140000的主要膜多肽，其氨基酸组成与在所有脊椎动物中都能找到的分子量为26000的主要内在膜蛋白完全不同。尽管140000的鱿鱼膜多肽不能被EDTA提取，但它最好被归类为外在蛋白，因为它具有高极性（主要是酸性残基），并且在对鱿鱼晶状体膜进行胰蛋白酶处理后可被完全降解。
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