Re T A, Loehr R F, Rodwell D E, D'Aleo C J, Burnett C M
Fundam Appl Toxicol. 1981 Nov-Dec;1(6):421-5. doi: 10.1016/s0272-0590(81)80021-8.
Para-phenylenediamine (PPD) was administered by gavage to pregnant Sprague-Dawley Rats at dose levels of 5, 10, 15, 20, and 30 mg/kg/day on days 6 through 15 of gestation (day 0 = day sperm was found in the vagina). A sham control group and a pair fed control group were studied at the same time. Pregnant animals were killed on day 20 of gestation and 1/3 of the fetuses were examined for visceral malformations and 2/3 for skeletal malformations and variations. Significant reductions in food consumption and weight gain were noted in the 30 mg/kg and pair fed control groups. Two pregnant rats given PPD at 30 mg/kg/day died but there were no deaths in any other dose groups. Fetal evaluations showed no biologically or statistically significant increase in malformations or developmental variations in any group. Therefore, although maternal toxicity was demonstrated at the two highest dose levels, there was no evidence of teratogenic or other embryotoxic effects.
在妊娠第6至15天（第0天=在阴道中发现精子的那天），通过灌胃给怀孕的斯普拉格-道利大鼠施用对苯二胺（PPD），剂量水平分别为5、10、15、20和30毫克/千克/天。同时研究了假手术对照组和配对喂食对照组。在妊娠第20天处死怀孕动物，1/3的胎儿检查内脏畸形，2/3检查骨骼畸形和变异。在30毫克/千克组和配对喂食对照组中，食物消耗和体重增加显著减少。两只给予30毫克/千克/天PPD的怀孕大鼠死亡，但其他剂量组均无死亡。胎儿评估显示，任何组的畸形或发育变异在生物学或统计学上均无显著增加。因此，尽管在两个最高剂量水平显示出母体毒性，但没有致畸或其他胚胎毒性作用的证据。
