Sieber-Blum M, Kahn C R
Stem Cells (1981). 1982 Nov-Dec;2(6):344-53.
Quail neural crest cells in vitro give rise to melanocytes and to adrenergic and cholinergic neurons. By supplementing the culture medium with heart cell conditioned medium (HCM), we stimulated cholinergic differentiation as indicated by a significant increase in the specific activity of the enzyme choline acetyltransferase. Melanin and catecholamine synthesis were concomitantly blocked as indicated by a lack of melanin granules and catecholamine-specific histofluorescence. Inhibition of pigmentation was fully reversible for at least 8 days, whereas inhibition of adrenergic differentiation became irreversible when cultures were exposed to HCM for more than 2 days. HCM also promoted cell attachment and spreading on the collagen substratum. These results suggest that HCM affected differentiation of quail neural crest cell progeny.
鹌鹑神经嵴细胞在体外可分化为黑素细胞、肾上腺素能神经元和胆碱能神经元。通过在培养基中添加心脏细胞条件培养基（HCM），我们刺激了胆碱能分化，这表现为胆碱乙酰转移酶的比活性显著增加。如缺乏黑色素颗粒和儿茶酚胺特异性组织荧光所示，黑色素和儿茶酚胺合成同时受到阻断。色素沉着的抑制至少在8天内是完全可逆的，而当培养物暴露于HCM超过2天时，肾上腺素能分化的抑制变得不可逆。HCM还促进细胞在胶原基质上的附着和铺展。这些结果表明，HCM影响了鹌鹑神经嵴细胞后代的分化。
