Sułowska Z, Tchórzewski H, Wojdalska B
Acta Physiol Pol. 1982 Sep-Dec;33(5-6):651-9.
Protein substances stimulating lymphocytes in vivo and in vitro are present in the lysosomes and secondary granules of neutrophil granulocytes. The mechanism of this stimulation is probably dependent on blockade of T suppressor cell functions. These observations stimulated the present study undertaken to clarify the effect of granulocyte factors on the multipotential stem cells of the haemopoietic system in mice subjected to sublethal irradiation. The experiments were carried out on F1 hybrid (Balb/cx NMRI) mice receiving a whole body dose of 3 Gy irradiation. Granulocyte proteins were injected intravenously in doses of 6 micrograms per one mouse 18, 48 and 72 hours after irradiation. The assayed proteins increased the number of macroscopic and microscopic colony forming cells in the mice spleens. The increase of erythrocyte granulocyte index in colonies and decrease of lymphocyte granulocyte index in peripheral blood have been observed. In the light of these results it is concluded that lysosomal granules and secondary granules of neutrophils contain protein factors stimulating the regeneration of haemopoietic cells of the erythrocyte and granulocyte series in the irradiated mice spleens.
刺激淋巴细胞体内和体外活性的蛋白质物质存在于中性粒细胞的溶酶体和二级颗粒中。这种刺激机制可能依赖于对T抑制细胞功能的阻断。这些观察结果激发了本研究，旨在阐明粒细胞因子对亚致死剂量照射小鼠造血系统多能干细胞的影响。实验在接受全身3 Gy照射剂量的F1杂种（Balb/c×NMRI）小鼠身上进行。照射后18、48和72小时，以每只小鼠6微克的剂量静脉注射粒细胞蛋白。检测的蛋白质增加了小鼠脾脏中宏观和微观集落形成细胞的数量。观察到集落中红细胞粒细胞指数增加，外周血中淋巴细胞粒细胞指数降低。根据这些结果得出结论，中性粒细胞的溶酶体颗粒和二级颗粒含有刺激受照射小鼠脾脏中红细胞和粒细胞系列造血细胞再生的蛋白质因子。
