伏隔核中的胆碱能作用：多巴胺和乙酰胆碱释放的调节。
Cholinergic action in the nucleus accumbens: modulation of dopamine and acetylcholine release.
作者信息
de Belleroche J S, Gardiner I M
出版信息
Br J Pharmacol. 1982 Feb;75(2):359-65. doi: 10.1111/j.1476-5381.1982.tb08794.x.
The action of oxotremorine and acetylcholine on the release of dopamine and acetylcholine from tissue slices of the rat nucleus accumbens was studied. Oxotremorine significantly enhanced the release of [14C]-dopamine evoked by 34 mMK+ and the EC50 for this action was 1.5 X 10(-7)M. A maximal enhancement (30%) for this effect was reached at 2 X 10(-7)M oxotremorine. A further enhancement of dopamine release occurred at concentrations of oxotremorine greater than 10(-4)M. The action of oxotremorine on [14C]-dopamine release was calcium-dependent and blocked by atropine (10(-4) M) but not mecamylamine (up to 10(-4) M). Oxotremorine affected [3H]-acetylcholine release differentially, inhibiting the K+-evoked release of [3H]-acetylcholine at concentrations greater than 10(-5) M. The IC50 for this process was 4.3 X 10(-5) M. Acetylcholine (8 X 10(-4) M) showed a similar pattern of action to oxotremorine: it enhanced the K+-evoked release of [14C]-dopamine (50%) and inhibited the K+-evoked release of [3H]-acetylcholine (30%). The mechanism of action of oxotremorine on dopamine release is discussed in terms of a presynaptic receptor-mediated process.
研究了氧化震颤素和乙酰胆碱对大鼠伏隔核组织切片中多巴胺和乙酰胆碱释放的作用。氧化震颤素显著增强了34 mM K⁺诱发的[¹⁴C] -多巴胺的释放，此作用的半数有效浓度（EC50）为1.5×10⁻⁷ M。在2×10⁻⁷ M氧化震颤素时达到该效应的最大增强（30%）。当氧化震颤素浓度大于10⁻⁴ M时，多巴胺释放进一步增强。氧化震颤素对[¹⁴C] -多巴胺释放的作用依赖于钙，并被阿托品（10⁻⁴ M）阻断，但不被美加明（高达10⁻⁴ M）阻断。氧化震颤素对[³H] -乙酰胆碱释放的影响不同，在浓度大于10⁻⁵ M时抑制K⁺诱发的[³H] -乙酰胆碱释放。此过程的半数抑制浓度（IC50）为4.3×10⁻⁵ M。乙酰胆碱（8×10⁻⁴ M）表现出与氧化震颤素相似的作用模式：它增强了K⁺诱发的[¹⁴C] -多巴胺释放（50%）并抑制了K⁺诱发的[³H] -乙酰胆碱释放（30%）。从突触前受体介导的过程角度讨论了氧化震颤素对多巴胺释放的作用机制。
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