Haag J R, Tempini A, Tschopp M, Haab P
J Physiol (Paris). 1982;78(9):843-7.
imultaneous analysis of a blood sample for its O2 and CO contents (Co2 and Cco) with the original manometric technique of Van Slyke entails a directional error on Co2 and Cco when blood contains CO. This important drawback is due to the fact that Na2S2O4 employed for the O2 absorption also reduces methemoglobin into hemoglobin which then reabsorbs a variable amount of the extracted CO. This amount is equal to the directional error and results in an overestimation of Co2 and in an underestimation of Cco. In order to analyze and possibly correct for this error, we have measured it by gas chromatography by determining Cco on blood samples with and without O2 absorption by Na2S2O4. The error has been found to be a function of both blood CO and Hb contents. An equation and a nomogram are presented allowing correction of the results obtained by the Van Slyke method. So corrected the results are equally precise in presence and in absence of CO (+/- 0.1 volume %) which makes simultaneous Co2 and Cco measurements with this classical and unexpensive method quite acceptable.
采用范斯莱克（Van Slyke）原始的测压技术同时分析血样中的氧含量（O₂）和一氧化碳含量（Co₂和Cco）时，当血液中含有一氧化碳时，Co₂和Cco会出现方向误差。这一重要缺陷是由于用于吸收O₂的Na₂S₂O₄也会将高铁血红蛋白还原为血红蛋白，然后血红蛋白会重新吸收一定量提取出的CO。这个量等于方向误差，导致Co₂被高估，Cco被低估。为了分析并可能校正这个误差，我们通过气相色谱法进行测量，通过测定用Na₂S₂O₄吸收O₂和未吸收O₂的血样中的Cco来测量该误差。已发现该误差是血液中CO含量和血红蛋白（Hb）含量的函数。给出了一个方程和一张列线图，用于校正通过范斯莱克方法获得的结果。经过这样校正后，无论是否存在CO（±0.1体积%），结果都同样精确，这使得用这种经典且成本低廉的方法同时测量Co₂和Cco相当可行。
