Chalikian D M, Barchi R L
Neurology. 1980 Mar;30(3):277-85. doi: 10.1212/wnl.30.3.277.
Previous electron spin resonance studies in myotonic muscular dystrophy (MyMD) have suggested that an increased cell surface membrane "fluidity" might underlie the widespread abnormalities in this disorder. We attempted to confirm and expand those findings by using another physical probe technique: fluorescence polarization spectroscopy. Spectral characteristics and rotational relaxation rates of four hydrophobic fluorescent probes were monitored in control and MyMD erythrocyte membrane ghosts. Structural unrelated probes were used to minimize the possibility that an alteration in physical properties in a restricted membrane microdomain would pass undetected. All probes were studied between 5 degrees C and 40 degrees C in each preparation to quantitate temperature-dependent changes. We failed to confirm the abnormality in apparent membrane fluidity previously reported in MyMD.
先前针对强直性肌营养不良（MyMD）的电子自旋共振研究表明，细胞表面膜“流动性”增加可能是该疾病广泛异常的基础。我们试图通过使用另一种物理探针技术：荧光偏振光谱法来证实并扩展这些发现。在对照和MyMD红细胞膜空壳中监测了四种疏水性荧光探针的光谱特征和旋转弛豫速率。使用与结构无关的探针，以尽量减少在受限膜微区中物理性质的改变未被检测到的可能性。在每个制剂中，于5摄氏度至40摄氏度之间研究所有探针，以定量温度依赖性变化。我们未能证实先前报道的MyMD中明显的膜流动性异常。
