Jakobsen A L, Chemnitz J
Acta Pathol Microbiol Scand A. 1980 Mar;88(2):119-24. doi: 10.1111/j.1699-0463.1980.tb02475.x.
In suspension cultures of JB-1-E cells approximately 11% of the cells contained a single large vacuole ("balloon cells") or signet-ring cells). Such cells were rarely observed in monolayer cultures, where the cells were attached to a substrate. Only a few "balloon cells" were observed in suspension cultures of HeLa S3 cells. Both cell lines were able to phagocytize latex particles of 5.7 micrometer mean diameter. Nocodazole significantly reduced the formation of "balloon cells" in suspension cultures of JB-1-E cells. Transmission electron microscopy indicated that various cytoplasmic membrane components probably could contribute to the membrane material of the vacuoles. It is believed that the formation of "balloon cells" is due to changes in the regulation of the cellular membrane material.
在JB - 1 - E细胞的悬浮培养物中，约11%的细胞含有单个大液泡（“气球样细胞”）或印戒细胞。在单层培养物（细胞附着于基质）中很少观察到此类细胞。在HeLa S3细胞的悬浮培养物中仅观察到少数“气球样细胞”。两种细胞系均能够吞噬平均直径为5.7微米的乳胶颗粒。诺考达唑显著减少了JB - 1 - E细胞悬浮培养物中“气球样细胞”的形成。透射电子显微镜显示，各种细胞质膜成分可能有助于液泡的膜材料形成。据信，“气球样细胞”的形成是由于细胞膜材料调节的变化。
