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利用C、Q和H显带技术分析铜绿蝇(维德曼)(双翅目:丽蝇科)的Y染色体重排

C, Q and H-banding in the analysis of Y chromosome rearrangements in Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae).

作者信息

Bedo D G

出版信息

Chromosoma. 1980;77(3):299-308. doi: 10.1007/BF00286055.

DOI:10.1007/BF00286055
PMID:7189455
Abstract

In Lucilia cuprina C-banding produces procentric bands on all autosomes and deep staining over most of the X and Y chromosomes which conciderably facilitates the analysis of complex Y chromosome rearrangements. The Y chromosome is generally darkly C-banded throughout while in the X chromosome a pale staining segment is found in the distal portion of the long arm. Modulation of the banding reaction results in 'grey' areas in both X and Y. When C-banding is compared with allocycly it is clear that not all heteropycnotic regions in the sex chromosomes C-band to the same extent. Secondary constrictions in the short arms of both X and Y chromosomes are clearly revealed by C-banding, the X satellite being polymorphic for size.--Q-banding results in a brightly fluorescing band in the short arm of structurally normal Y chromosomes. This band loses its fluorescence in some translocations, probably through a position effect. Hoechst 33258 staining does not produce any brightly fluorescing bands.

摘要

在铜绿蝇中,C 带在所有常染色体上产生着丝粒带,并且在大部分 X 和 Y 染色体上染色很深,这极大地促进了对复杂 Y 染色体重排的分析。Y 染色体通常在整个长度上 C 带染色很深,而在 X 染色体中,在长臂的远端部分发现一个浅染区段。带型反应的调节会在 X 和 Y 染色体上产生“灰色”区域。当将 C 带与异固缩进行比较时,很明显性染色体中并非所有异固缩区域的 C 带染色程度都相同。C 带清楚地显示出 X 和 Y 染色体短臂中的次缢痕,X 随体在大小上具有多态性。——Q 带在结构正常的 Y 染色体短臂中产生一条明亮荧光带。在一些易位中,这条带会失去荧光,可能是由于位置效应。Hoechst 33258 染色不会产生任何明亮荧光带。

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本文引用的文献

1
Polytene chromosomes and linkage group-chromosome correlations in the Australian sheep blowfly Lucilia cuprina (Diptera: Calliphoridae).澳大利亚羊蝇 Lucilia cuprina(双翅目:丽蝇科)的多线染色体与连锁群 - 染色体相关性
Chromosoma. 1969;26(2):208-14. doi: 10.1007/BF00326456.
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Changes in the fluorescence patterns of translocated Y chromosome segments in Drosophila melanogaster.黑腹果蝇中易位Y染色体片段荧光模式的变化。
Chromosoma. 1971;34(3):274-80. doi: 10.1007/BF00286153.
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Quinacrine, a chromosome stain specific for deoxyadenylate-deoxythymidylaterich regions in DNA.
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4
Genetic instability in mass-rearing colonies of a sex-linked translocation strain of Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae) during a field trial of genetic control.在性连锁易位品系丽蝇蛹集金小蜂(Wiedemann)(双翅目:Calliphoridae)的田间试验中遗传控制过程中大规模繁殖群体的遗传不稳定性。
Theor Appl Genet. 1980 May;58(3-4):169-75. doi: 10.1007/BF00263113.
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The use of bridging systems to increase genetic variability in compound chromosome strains for genetic control of Lucilia cuprina (Wiedemann).利用桥接系统增加复合染色体品系的遗传变异性,以实现对丽蝇蛹集金小蜂(Wiedemann)的遗传控制。
Theor Appl Genet. 1982 Dec;63(4):295-305. doi: 10.1007/BF00303899.
6
Field trial of a compound chromosome strain for genetic control of the sheep blowfly Lucilia cuprina.复合染色体品系对绵羊狂蝇 Lucilia cuprina 的遗传控制的田间试验。
Theor Appl Genet. 1985 Apr;70(1):13-21. doi: 10.1007/BF00264477.
7
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Chromosome Res. 1994 May;2(3):191-9. doi: 10.1007/BF01553319.
8
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9
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