[Effect of semicarbazide on the electrical and metabolic activity of cerebellar and visual cortex neurons in tissue culture].

The influence of semicarbazide (SC), an inhibitor of glutamate decarboxylase (GDC), on electrical and respiratory activity of the Purkinje cells and cortical neurons of mice was investigated in tissue culture. A biphase character of the SC influence on the investigated parameters was demonstrated. The first excitatory phase could be produced by the blocking action of SC on the GDC synthesis. It is suggested that the prevalence of the excitatory background under conditions of blockade of the GABAergic system led to strong depolarization of the nerve cell membrane, evoking the secondary blocking effect under SC influence. The influence of SC was more expressed on the Purkinje cells than on neurons of the visual cortex, which testifies to a higher content of GDC in inhibitory synapses of cerebellar cells and a more active synthesis of GABA there. For both structures a period was discovered (10-14 days of development in tissue culture) when the biphase effect of SC was maximal. The early formation of GABA-inhibitory synapses in the investigated structures of the brain in the ontogenetic development is suggested.