Vlasova I G, Kostiaeva G N, Luk'ianova L D
Neirofiziologiia. 1980;12(2):138-45.
The influence of semicarbazide (SC), an inhibitor of glutamate decarboxylase (GDC), on electrical and respiratory activity of the Purkinje cells and cortical neurons of mice was investigated in tissue culture. A biphase character of the SC influence on the investigated parameters was demonstrated. The first excitatory phase could be produced by the blocking action of SC on the GDC synthesis. It is suggested that the prevalence of the excitatory background under conditions of blockade of the GABAergic system led to strong depolarization of the nerve cell membrane, evoking the secondary blocking effect under SC influence. The influence of SC was more expressed on the Purkinje cells than on neurons of the visual cortex, which testifies to a higher content of GDC in inhibitory synapses of cerebellar cells and a more active synthesis of GABA there. For both structures a period was discovered (10-14 days of development in tissue culture) when the biphase effect of SC was maximal. The early formation of GABA-inhibitory synapses in the investigated structures of the brain in the ontogenetic development is suggested.
在组织培养中研究了谷氨酸脱羧酶（GDC）抑制剂氨基脲（SC）对小鼠浦肯野细胞和皮质神经元电活动及呼吸活动的影响。结果表明SC对所研究参数的影响具有双相性。第一个兴奋期可能是由于SC对GDC合成的阻断作用所致。有观点认为，在GABA能系统被阻断的情况下，兴奋背景占优势导致神经细胞膜强烈去极化，在SC的影响下引发继发性阻断效应。SC对浦肯野细胞的影响比对视觉皮层神经元的影响更明显，这证明小脑细胞抑制性突触中GDC含量更高，且那里GABA的合成更活跃。对于这两种结构，都发现了一个时期（组织培养中发育10 - 14天），此时SC的双相效应最大。这表明在所研究的脑结构中，GABA抑制性突触在个体发育过程中形成较早。
