Kanda N, Kato H
Chromosoma. 1980;78(1):113-21. doi: 10.1007/BF00291910.
Differential staining of sister chromatids in male mouse meiotic cells was achieved by continuous infusion with BrdU from the tail vein followed by Giemsa staining (the FPG technique of Perry and Wolff, 1974). Analysis of 341 bivalents including XY, and 21 univalents reveals that: (1) visible crossing over coincided exactly with the site of chiasmata; (2) no evidence was obtained in support of chiasma terminalisation; (3) an anomalous type of crossing over was found in the monochiasmatic bivalents, which could not be explained by the conventional hypothesis for crossing over; (4) some of the terminally associated bivalents might be achiasmatic, and univalents might have originated from such bivalents; (5) in XY bivalents, sister chromatid association was between lightly and darkly stained chromatids, suggesting a lack of crossing over and the existence of other genetic controls for this association; (6) during meiosis sister chromatid exchanges (SCEs) might be less frequent than crossovers.
通过从尾静脉持续注入5-溴脱氧尿嘧啶核苷（BrdU），随后进行吉姆萨染色（Perry和Wolff于1974年提出的FPG技术），实现了雄性小鼠减数分裂细胞中姐妹染色单体的差异染色。对包括XY在内的341个二价体和21个单价体的分析表明：（1）可见的交叉互换与交叉点的位置完全一致；（2）未获得支持交叉点端化的证据；（3）在单交叉二价体中发现了一种异常类型的交叉互换，这无法用传统的交叉互换假说来解释；（4）一些末端相连的二价体可能是无交叉点的，单价体可能起源于此类二价体；（5）在XY二价体中，姐妹染色单体的关联存在于浅染和深染的染色单体之间，这表明缺乏交叉互换以及存在其他对此关联的遗传控制；（6）在减数分裂过程中，姐妹染色单体交换（SCEs）可能比交叉互换的频率更低。
