Greven J, Beckers M, Defrain W, Meywald K, Heidenreich O
Pflugers Arch. 1980 Mar;384(1):61-4. doi: 10.1007/BF00589515.
The effect of the non-diuretic dextrorotatory isomer of ozolinone on furosemide-induced diuresis was studied by means of clearance and micropuncture techniques in rats. After intravenous injection, d-ozolinone antagonized the furosemide-induced increase in renal fluid and electrolyte excretion in a dose-related manner. Microperfusion experiments of Henle's loop in vivo revealed that d-ozolinone did not interfere with the action of furosemide at this tubular site. However, d-ozolinone markedly decreased the furosemide to inulin clearance ratio, presumably as a consequence of inhibition of furosemide secretion into the proximal tubules. It is assumed that, in consequence of a high affinity for the proximal organic acid transport system, d-ozolinone depresses proximal tubular furosemide secretion and prevents transfer of this diuretic to the tubular fluid. Thus, under the influence of d-ozolinone, furosemide cannot reach the loop of Henle in sufficient amounts and its diuretic effect is blocked.
采用清除率和微穿刺技术，在大鼠身上研究了奥佐利酮的非利尿右旋异构体对呋塞米诱导利尿作用的影响。静脉注射后，d-奥佐利酮以剂量相关的方式拮抗呋塞米诱导的肾液体和电解质排泄增加。体内对亨氏袢进行微灌注实验表明，d-奥佐利酮在此肾小管部位不干扰呋塞米的作用。然而，d-奥佐利酮显著降低了呋塞米与菊粉的清除率比值，推测这是由于抑制了呋塞米向近端小管的分泌。据推测，由于对近端有机酸转运系统具有高亲和力，d-奥佐利酮会抑制近端小管呋塞米的分泌，并阻止这种利尿剂转移到肾小管液中。因此，在d-奥佐利酮的影响下，呋塞米无法足量到达亨氏袢，其利尿作用被阻断。
