Knehr H E, Heinle H, Betz E
Pflugers Arch. 1980 Aug;387(1):73-7. doi: 10.1007/BF00580848.
In rabbit carotid arteries intimal smooth muscle cell proliferations were induced by repeated local electrical stimulation of the artery wall. Oxygen uptake and lactate production of the proliferating tissue were determined in vitro and compared with the results obtained from nonstimulated arterial wall segments. Under the incubation conditions employed, oxygen consumption and lactate production of the proliferating tissue were 91.3 and 62.5 nmol x min-1 x mg-1 DNA, compared with the control values of 70.8 and 41.6 nmol x min-1 x mg-1 DNA. The DNA content of the proliferates and normal arterial wall tissue amounted to 9.6 and 12.0 microgram DNA x mg-1 dry weight. The results show that in proliferates obtained with the described procedure there is an increased capacity for ATP regenerating systems, respiration and glycolysis, indicating a modified function of the proliferating smooth muscle cells.
在兔颈动脉中，通过对动脉壁进行反复局部电刺激诱导内膜平滑肌细胞增殖。在体外测定增殖组织的氧摄取和乳酸生成，并与未受刺激的动脉壁段所获得的结果进行比较。在所采用的孵育条件下，增殖组织的氧消耗和乳酸生成分别为91.3和62.5 nmol·min⁻¹·mg⁻¹ DNA，而对照值分别为70.8和41.6 nmol·min⁻¹·mg⁻¹ DNA。增殖组织和正常动脉壁组织的DNA含量分别为9.6和12.0微克DNA·mg⁻¹干重。结果表明，用所述方法获得的增殖组织中，ATP再生系统、呼吸和糖酵解的能力增强，这表明增殖的平滑肌细胞功能发生了改变。
