Horisberger M
Physiol Chem Phys. 1980;12(3):195-204.
Adhesion of glutaraldehyde-fixed human red blood cells (RBC) to polystyrene was measured as a function of concentration of mono-, di-, and trivalent cations. The effectiveness for preventing adhesion was trivalent > divalent > monovalent. Adhesion increased with ionic strength but no specificity of cations in a particular valency group was observed. Adhesion as a function of NaCl concentration was not affected by temperature between 1 degrees C and 22 degrees C but decreased in 150 mM NaCl above pH 6. Chicken RBC and neuraminidase-treated human RBC were more adhesive than untreated human RBC in NaCl. In 150 mM NaCl, adhesion of human RBC decreased in the presence of hemolyzate or polyethylene glycols (mol. wt. 10,000-300,000). These results support the hypothesis that RBC adhesion to polystyrene occurs in the primary minimum of the potential energy of interaction and is governed by attractive London-Van der Waals and repulsive electrostatic forces. The implications were applied to study of hemagglutination of RBC with lectin and lectin-labeled gold granules in polystyrene microplates.
测定了戊二醛固定的人红细胞（RBC）与聚苯乙烯的黏附情况，该黏附情况是单价、二价和三价阳离子浓度的函数。防止黏附的有效性为三价>二价>单价。黏附随离子强度增加，但在特定价态组中未观察到阳离子的特异性。作为NaCl浓度函数的黏附不受1℃至22℃之间温度的影响，但在pH 6以上的150 mM NaCl中会降低。在NaCl中，鸡红细胞和神经氨酸酶处理的人红细胞比未处理的人红细胞更具黏附性。在150 mM NaCl中，人红细胞的黏附在存在溶血产物或聚乙二醇（分子量10,000 - 300,000）时会降低。这些结果支持了这样的假设，即红细胞与聚苯乙烯的黏附发生在相互作用势能的第一极小值处，并受吸引性的伦敦 - 范德华力和排斥性的静电力控制。这些结论被应用于研究聚苯乙烯微孔板中红细胞与凝集素以及凝集素标记的金颗粒的血凝反应。
