Grande M
Biomedicine. 1977 Oct;26(5):326-30.
A cytochemical investigation was carried out on the cells that make up the clusters forming in agar cultures of normal human bone marrow after 3, 4, and 5 days of incubation. All the clusters were found to comprise young monocytes that were PAS, peroxidase, Sudan Black, and alpha-naphthyl and naphthol-ASD-acetate esterase positive. Lysozyme activity, investigated by the cytobacterial test, was absent in all cases. After 3 days of incubation a large number of pairs of lymphomonocytes or monocytes with the same cytochemical characteristics were observed. When a method of double esterase incubation (alpha-naphthyl or naphthol-ASD-acetate and naphthol-ASD-chloroacetate) was performed on the same slide, no cells of the granulocytic line or intermediate cells between these and the monocytes were observed in any of the clusters. These results indicate that there is direct monocytic differentiation of the colony-forming cells cultured. The origins and stages observed in the maturation of the monocytes are discussed.
对正常人骨髓琼脂培养物在孵育3天、4天和5天后形成的细胞簇中的细胞进行了细胞化学研究。发现所有细胞簇均由年轻单核细胞组成，这些单核细胞对过碘酸雪夫反应（PAS）、过氧化物酶、苏丹黑以及α-萘酯和萘酚-ASD-醋酸酯酶呈阳性。通过细胞细菌试验研究的溶菌酶活性在所有情况下均未检测到。孵育3天后，观察到大量具有相同细胞化学特征的淋巴细胞单核细胞对或单核细胞对。当在同一张载玻片上进行双酯酶孵育方法（α-萘酯或萘酚-ASD-醋酸酯和萘酚-ASD-氯醋酸酯）时，在任何细胞簇中均未观察到粒细胞系细胞或这些细胞与单核细胞之间的中间细胞。这些结果表明培养的集落形成细胞存在直接的单核细胞分化。讨论了单核细胞成熟过程中观察到的起源和阶段。
