Hack G, Karzel K
Anaesthesist. 1981 Feb;30(2):88-94.
Cultures of a permanently in suspension growing line of Ehrlich ascites tumor cells (EATC) were studied regarding their use as a test system for the determination of cytotoxic effects of anaesthetics. These cells, having a comparatively high reduplication rate, are cultured at 37 degrees C in vitro in a chemically defined liquid medium supplemented by 15% horse serum without agitation in a closed system. In order to detect drug effects on the cultures various cellular parameters can be determined due to the suspension character of the cultures without complicated preparatory measures, e.g. cell number or multiplication rate, cell volume, cell volume distribution. Moreover biochemical parameters, such as protein or nucleic acid content, may be estimated after centrifugation of the cultures separately in the cell sediment and the medium supernatant. Applying drugs of various pharmacological groups (cytostatic and anti-inflammatory drugs, local and general anaesthetics) the usefulness of some of these parameters for the detection of cytotoxic effects was studied.
对艾氏腹水肿瘤细胞（EATC）的一个永久悬浮生长系的培养物进行了研究，以探讨其作为测定麻醉药细胞毒性作用的测试系统的用途。这些细胞具有相对较高的复制率，在体外于37℃的化学限定液体培养基中培养，该培养基添加了15%的马血清，在封闭系统中不搅拌。由于培养物的悬浮特性，无需复杂的预处理措施即可测定各种细胞参数，从而检测药物对培养物的影响，例如细胞数量或增殖率、细胞体积、细胞体积分布。此外，在分别对培养物进行离心后，可在细胞沉淀物和培养基上清液中估计生化参数，如蛋白质或核酸含量。应用各种药理组的药物（细胞抑制剂和抗炎药、局部和全身麻醉药），研究了其中一些参数用于检测细胞毒性作用的有效性。
