Bahr G F, Schell B E, Engler W F
Ultramicroscopy. 1981;6(3):251-8. doi: 10.1016/s0304-3991(81)80160-x.
Instrumental additions to the Zeiss EM-10 electron microscope are described as well as a flat-bed scanning densitometer specifically intended for the tomographic assessment of the three-dimensional structure of chromosomes. The additions to the electron microscope consist of two ion-getter pumps, reducing contamination to 0.0004 A/s. Further, a device was attached that made it possible to indicate through markers in the final negative the direction of the axis of tilt of the goniometer stage, i.e. markers indicating the tomographic axis in the digitized image. The flat-bed scanner permits correction of image rotation caused by refocusing. The preparation itself contains polystyrene latex spheres, i.e. objects the shape of which is independent of rotation (tilt). These can be used for computational corrections of the digitized image.
介绍了对蔡司EM - 10电子显微镜的仪器改进，以及一台专门用于断层扫描评估染色体三维结构的平板扫描密度计。电子显微镜的改进包括两个离子吸气泵，将污染降低到0.0004埃/秒。此外，还安装了一个装置，通过最终底片上的标记可以指示测角仪台的倾斜轴方向，即在数字化图像中指示断层扫描轴的标记。平板扫描仪可以校正由于重新聚焦引起的图像旋转。制备物本身包含聚苯乙烯乳胶球，即形状与旋转（倾斜）无关的物体。这些可用于数字化图像的计算校正。
