Samson A C, Chambers P, Lee C M, Simon E
J Gen Virol. 1981 May;54(Pt 1):197-201. doi: 10.1099/0022-1317-54-1-197.
Analysis of six temperature-sensitive (ts) mutants of Newcastle disease virus (NDV) representing each of six complementation groups by both SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis revealed that in only one mutant was there an alteration in the isoelectric point of a protein. This altered protein was the nucleocapsid-associated protein, NAP. In addition, the mobility of the haemagglutinin-neuraminidase protein, HN, was decreased on non-reduced SDS-PAGE in this mutant. All independent ts+ clones derived from this mutant had normal NAP but HN protein migrated at the decreased rate. Haemagglutinating activity of wide-type (ts+) and ts virions was equally thermostable. Wild-type and ts+ clones derived from this ts mutant were RNA(+) at both permissive and non-permissive temperatures, whereas the ts mutant was RNA(-) at the non-permissive temperature. This ts mutant appears to be a double mutant in both HN and NAP genes, the latter only being a temperature-sensitive lesion which affects virus-directed RNA synthesis.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和二维凝胶电泳对代表六个互补群的新城疫病毒（NDV）的六个温度敏感（ts）突变体进行分析，结果显示只有一个突变体中的一种蛋白质的等电点发生了改变。这种改变的蛋白质是核衣壳相关蛋白（NAP）。此外，在该突变体中，非还原SDS-PAGE上血凝素-神经氨酸酶蛋白（HN）的迁移率降低。从该突变体衍生的所有独立ts+克隆都具有正常的NAP，但HN蛋白以降低的速率迁移。野生型（ts+）和ts病毒粒子的血凝活性同样耐热。从该ts突变体衍生的野生型和ts+克隆在允许温度和非允许温度下均为RNA（+），而该ts突变体在非允许温度下为RNA（-）。这个ts突变体似乎在HN和NAP基因中都是双突变体，后者只是一个影响病毒指导的RNA合成的温度敏感损伤。
