Rexroad C E
J Anim Sci. 1981 Oct;53(4):1070-6. doi: 10.2527/jas1981.5341070x.
Two experiments were conducted to study the role of estradiol and progesterone in regulating steroid binding sites in sheep uterine myometrium. Exogenous estradiol increased the concentration of estradiol binding sites in nuclei and subsequently increased the total cell content of estradiol binding sites. Estradiol increased the number of progestogen binding sites (promegestone binding) and may have enhanced the translocation of progestogen binding sites to nuclei. Progesterone decreased the number of progestogen binding sites and blocked estradiol-induced increase in cytoplasmic estradiol binding sites. Progesterone did not block the estradiol-induced increase in estradiol binding sites in nuclei. Estradiol increases myometrial RNA to DNA ratios with in 24 hr of a single injection, whereas progesterone did not. Progesterone blocked the estradiol-induced increase in RNA:DNA. Estradiol apparently promotes the synthesis of both types of binding site. Progesterone, on the other hand, appears to inhibit estrogen-induced synthesis of steroid binding sites by blocking estradiol action at the nucleus. Progesterone may also decrease the number of progestogen binding site through a mechanism other than its "anti-estrogenic" action.
进行了两项实验，以研究雌二醇和孕酮在调节绵羊子宫肌层类固醇结合位点中的作用。外源性雌二醇增加了细胞核中雌二醇结合位点的浓度，随后增加了雌二醇结合位点的细胞总含量。雌二醇增加了孕激素结合位点的数量（孕美雌酮结合），并可能增强了孕激素结合位点向细胞核的转运。孕酮减少了孕激素结合位点的数量，并阻断了雌二醇诱导的细胞质中雌二醇结合位点的增加。孕酮并未阻断雌二醇诱导的细胞核中雌二醇结合位点的增加。单次注射雌二醇后24小时内，子宫肌层的RNA与DNA比值增加，而孕酮则没有。孕酮阻断了雌二醇诱导的RNA:DNA增加。雌二醇显然促进了两种结合位点的合成。另一方面，孕酮似乎通过阻断雌二醇在细胞核的作用来抑制雌激素诱导的类固醇结合位点的合成。孕酮也可能通过其“抗雌激素”作用以外的机制减少孕激素结合位点的数量。
