内源性S-腺苷甲硫氨酸对红细胞中儿茶酚-O-甲基转移酶活性测定的影响。
Influence of endogenous S-adenosylmethionine on the determination of catechol O-methyltransferase activity in red blood cells.
作者信息
Hoberg E, Hempel K
出版信息
Clin Chim Acta. 1978 Dec 1;90(2):107-14. doi: 10.1016/0009-8981(78)90511-9.
A double isotope method for the simultaneous determination of S-adenosylmethionine (SAM) and catechol O-methyltransferase (COMT) activity in red blood cells was developed. Healthy persons contained 7.9 +/- 1.9 nmol SAM per ml red blood cells and 1.9 to 3.8 nmol per ml plasma. Epinephrine increases the SAM biosynthesis rate in red blood cells. The possible influence of endogenous SAM on previous methods to determine COMT activity in red blood cells is discussed. The statement of Briggs and Briggs (Briggs, M.H. and Briggs, M. (1973) Experientia 29, 278--281) that COMT activity in red blood cells is lower during the last 3 months of pregnancy could not be verified by us.
开发了一种用于同时测定红细胞中S-腺苷甲硫氨酸（SAM）和儿茶酚-O-甲基转移酶（COMT）活性的双同位素方法。健康人每毫升红细胞中含有7.9±1.9 nmol SAM，每毫升血浆中含有1.9至3.8 nmol。肾上腺素可提高红细胞中SAM的生物合成速率。讨论了内源性SAM对先前测定红细胞中COMT活性方法的可能影响。Briggs和Briggs（Briggs，M.H.和Briggs，M.（1973）实验医学 29，278 - 281）关于妊娠最后3个月红细胞中COMT活性较低的说法未得到我们的证实。
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