Aubert C, Luccioni C, Coassolo P, Sommadossi J P, Cano J P
Arzneimittelforschung. 1981;31(12):2048-53.
Gas chromatography-mass spectrometry (GC/MS) and high performance liquid chromatography (HPLC) procedures have developed to determine 5-fluorouracil (5-FU) in human plasmas. Internal standard in both systems was 5-bromouracil (5-BrU). The HPLC assay used an extracted plasma with an ether-isopropanol mixture (75-25): the analyses were performed on a mu Porasil column (10 micrometer) with hexane-ethanol (75-25) as elution solvent (detection limit 50 ng/ml). For GC/MS, after precipitation of proteins, the plasma was purified through a microcolumn packed with AG1 X 4 resin, and then the components were methylated. Electron impact (EI) and selective ion monitoring (SIM) mode was retained for GC/MS quantitative studies (detection limit 5 ng/ml). Moreover, the authors compared these two techniques by measurements on plasma samples obtained from patients after rapid i.v. injections, infusions and p.o. administration.
气相色谱-质谱联用（GC/MS）和高效液相色谱（HPLC）方法已被开发用于测定人血浆中的5-氟尿嘧啶（5-FU）。两个系统中的内标均为5-溴尿嘧啶（5-BrU）。HPLC分析采用乙醚-异丙醇混合物（75-25）萃取血浆，分析在μ Porasil柱（10微米）上进行，以正己烷-乙醇（75-25）作为洗脱溶剂（检测限为50纳克/毫升）。对于GC/MS，蛋白质沉淀后，血浆通过填充AG1 X 4树脂的微柱进行纯化，然后对各成分进行甲基化处理。GC/MS定量研究采用电子轰击（EI）和选择性离子监测（SIM）模式（检测限为5纳克/毫升）。此外，作者通过对快速静脉注射、输注和口服给药后患者血浆样本的测量比较了这两种技术。
