Barakett L, Pillay D T
Gerontology. 1982;28(1):1-9. doi: 10.1159/000212505.
Polysomes and their associated ribonucleic acids were extracted from ageing soybean cotyledons. Density gradient analysis showed that the ribosomes of older cotyledons were present largely as monosomes and light polysomes while younger cotyledons possessed a high proportion of heavy polysomes. Increased polysomal and mRNA activities were observed in 3- to 5-day-old and again in 10-day-old cotyledons. The presence of polyadenylated mRNA was determined by oligo (dT) cellulose chromatography and confirmed by hybridization with 3H-polyuridylic acid.
从老化的大豆子叶中提取了多核糖体及其相关的核糖核酸。密度梯度分析表明，较老的子叶中的核糖体主要以单体和轻多核糖体的形式存在，而较年轻的子叶中重多核糖体的比例较高。在3至5日龄以及10日龄的子叶中观察到多核糖体和mRNA活性增加。通过寡聚（dT）纤维素色谱法测定了聚腺苷酸化mRNA的存在，并通过与3H-聚尿苷酸杂交进行了证实。
