Akiyama K, Sato M, Otsuki S
Biol Psychiatry. 1982 Feb;17(2):223-31.
The specific 3H-spiperone binding to membrane homogenates of the striatum, mesolimbic area, and frontal cortex was examined in two groups of rats pretreated once daily with saline or 4 mg/kg of methamphetamine (MAP) for 14 days. At 7 days following cessation of chronic pretreatment, all rats received an injection of 4 mg/kg of MAP and were decapitated 1 hr after the injection. In the chronic saline-pretreatment group, the single administration of MAP induced significant changes in the number (Bmax) of specific 3H-spiperone binding sites (a decrease in the striatum and an increase in the mesolimbic area and frontal cortex), but no significant changes in the affinity (KD) in any brain area. The chronic MAP pretreatment markedly augmented the changes in Bmax in the striatum and mesolimbic area. The increase in specific 3H-spiperone binding sites in the mesolimbic area is discussed in relation to MAP-induced behavioral hypersensitivity.
在两组大鼠中检测了特异性3H-螺哌隆与纹状体、中脑边缘区和额叶皮质膜匀浆的结合情况，这两组大鼠每天用生理盐水或4mg/kg甲基苯丙胺（MAP）预处理14天。在慢性预处理停止后7天，所有大鼠接受4mg/kg MAP注射，并在注射后1小时断头。在慢性生理盐水预处理组中，单次给予MAP导致特异性3H-螺哌隆结合位点数量（Bmax）发生显著变化（纹状体减少，中脑边缘区和额叶皮质增加），但在任何脑区的亲和力（KD）均无显著变化。慢性MAP预处理显著增强了纹状体和中脑边缘区Bmax的变化。结合MAP诱导的行为超敏反应讨论了中脑边缘区特异性3H-螺哌隆结合位点的增加。
