Willard K E
Clin Chem. 1982 Apr;28(4 Pt 2):1074-83.
Lymphocytes cultured with concentrated human urine develop many alterations (Clin. Chem. 27:1327, 1981). New proteins appear ("Urocon" proteins), while others disappear ("Urocof" proteins) from the two-dimensional gel pattern. This paper characterizes a human urinary leukocyte effector protein ("HULEP") that is responsible for the appearance of the Urocon:11-15 proteins in human lymphocytes. The effector molecule, HULEP-1, is a protein with a relative molecular mass in the range of 29000 to 43000. Urocon:11-15 production, cytoskeletal changes in the lymphocyte, and the presence of added HULEP-1 in the cultures are all directly related. This molecule appears to alter the structure of the lymphocyte by degrading actin and possible other lymphocyte cytoskeletal proteins. Peptide maps of Urocon:11-15 and beta and gamma actin provide evidence that Urocon:11-15 proteins are closely related to nonmuscle actin, and may be degradation products of this major cytoskeletal protein.
用浓缩人尿培养的淋巴细胞会发生许多变化（《临床化学》27:1327，1981年）。新的蛋白质出现（“尿蛋白”），而其他蛋白质则从二维凝胶图谱中消失（“尿共蛋白”）。本文描述了一种人尿白细胞效应蛋白（“人尿白细胞效应蛋白”），它导致人淋巴细胞中出现尿蛋白：11 - 15蛋白。效应分子人尿白细胞效应蛋白 - 1是一种相对分子质量在29000至43000范围内的蛋白质。尿蛋白：11 - 15的产生、淋巴细胞中的细胞骨架变化以及培养物中添加的人尿白细胞效应蛋白 - 1的存在都直接相关。该分子似乎通过降解肌动蛋白和可能的其他淋巴细胞细胞骨架蛋白来改变淋巴细胞的结构。尿蛋白：11 - 15以及β和γ肌动蛋白的肽图提供了证据，表明尿蛋白：11 - 15蛋白与非肌肉肌动蛋白密切相关，并且可能是这种主要细胞骨架蛋白的降解产物。
