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人类大脑蛋白质的二维凝胶电泳。II. 特定蛋白质与脑亚组分

Two-dimensional gel electrophoresis of human brain proteins. II. Specific proteins and brain subfractions.

作者信息

Comings D E, Carraway N G, Pekkula-Flagan A

出版信息

Clin Chem. 1982 Apr;28(4 Pt 2):790-7.

PMID:7200410
Abstract

Brain proteins extractable with distilled water or 9 mol/L urea were subjected to two-dimensional gel electrophoresis. They are examined in relation to the identification of actin, tubulin, neurofilament proteins, glial fibrillary acidic protein; proteins of human embryonic neocortex, synaptosomes, myelin, and plasma; and rat neocortex, rat glial, and mouse neuroblastoma cell lines.

摘要

可用蒸馏水或9摩尔/升尿素提取的脑蛋白进行二维凝胶电泳分析。对其进行检测,以鉴定肌动蛋白、微管蛋白、神经丝蛋白、胶质纤维酸性蛋白;人类胚胎新皮质、突触体、髓磷脂和血浆中的蛋白质;以及大鼠新皮质、大鼠神经胶质和小鼠神经母细胞瘤细胞系中的蛋白质。

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