Gwynne A, Condon W A
J Reprod Fertil. 1982 May;65(1):151-6. doi: 10.1530/jrf.0.0650151.
Exposure of bovine luteal tissue to the anti-microfilament agent cytochalasin B inhibited both basal and LH-induced steroidogenesis, but had no effect on the portion of the progesterone secreted into the incubation medium. Addition of 10% calf serum to the incubation medium caused no significant increase in progesterone synthesis, but produced a significant increase in the portion of the progesterone found in the incubation medium. This increased secretion in the serum-containing medium was not altered by the presence of LH, and cytochalasin B was unable to prevent it. Exposure of the luteal tissue to the antimicrotubular drugs, colchicine and vinblastine, produced no alteration in synthesis or secretion of progesterone, even when LH was present, and neither drug inhibited the serum-induced increase in secretion of progesterone. These studies suggest that luteal cell microfilaments may be involved in LH-stimulated progesterone production while microtubules apparently play no direct role in progesterone synthesis.
将牛黄体组织暴露于抗微丝剂细胞松弛素B会抑制基础和促黄体生成素（LH）诱导的类固醇生成，但对分泌到孵育培养基中的孕酮部分没有影响。向孵育培养基中添加10%的小牛血清不会使孕酮合成显著增加，但会使孵育培养基中发现的孕酮部分显著增加。含血清培养基中这种增加的分泌不受LH存在的影响，细胞松弛素B也无法阻止它。将黄体组织暴露于抗微管药物秋水仙碱和长春碱，即使存在LH，孕酮的合成或分泌也不会发生改变，并且这两种药物都不会抑制血清诱导的孕酮分泌增加。这些研究表明，黄体细胞微丝可能参与LH刺激的孕酮产生，而微管显然在孕酮合成中不发挥直接作用。
