Gregoraszczuk E L, Słomczyńska M
Department of Physiology, Jagiellonian University, Kraków, Poland.
Folia Histochem Cytobiol. 1996;34(1):35-9.
The involvement of microtubules (MT) and microfilaments (MF) in LH-regulation of luteal cell steroidogenesis was assessed at the middle stage of corpus luteum development. The influence of microtubule- and microfilament-altering agents on basal and LH-stimulated progesterone (P4) production and secretion into the incubation medium was determined by RIA. LH-stimulated P4 production was 2.5 times higher than in the control cultures. Cytochalasin B (Cyt B) was without effect on basal P4 synthesis but increased the basal fraction of P4 secreted into the incubation medium, while colchicine (Col) increased both basal P4 synthesis and the fraction of P4 secreted into the incubation medium. LH-stimulated progesterone synthesis was reduced by Col, but the fraction secreted into the incubation medium increased. Cyt B had no effect on LH-stimulated synthesis but it decreased the fraction of P4 secreted into the incubation medium. Our findings demonstrate significant differences in the effect of Cyt B and Col on steroidogenesis in corpus luteum. We conclude that microtubules play an important role in the process of LH-stimulated P4 synthesis, while microfilaments act in the process of basal and LH-stimulated P4 secretion.
在黄体发育中期，评估了微管（MT）和微丝（MF）在黄体细胞类固醇生成的促黄体生成素（LH）调节中的作用。通过放射免疫分析（RIA）确定了改变微管和微丝的试剂对基础和LH刺激的孕酮（P4）产生以及分泌到孵育培养基中的影响。LH刺激的P4产生比对照培养物高2.5倍。细胞松弛素B（Cyt B）对基础P4合成无影响，但增加了分泌到孵育培养基中的P4基础部分，而秋水仙碱（Col）增加了基础P4合成以及分泌到孵育培养基中的P4部分。Col降低了LH刺激的孕酮合成，但分泌到孵育培养基中的部分增加。Cyt B对LH刺激的合成无影响，但它降低了分泌到孵育培养基中的P4部分。我们的研究结果表明，Cyt B和Col对黄体类固醇生成的影响存在显著差异。我们得出结论，微管在LH刺激的P4合成过程中起重要作用，而微丝在基础和LH刺激的P4分泌过程中起作用。
