Komaniwa H, Fukusho A, Shimizu Y
Natl Inst Anim Health Q (Tokyo). 1981 Winter;21(4):153-8.
Hog cholera (HC) virus and antibody against it were estimated by the END method with microplates and CPK porcine kidney cell strain. To establish the technique of this method, studies were made on such basic conditions of the method as the type of strain of Newcastle disease virus (NDV), the time of challenge with this virus, and the concentration of serum in culture fluid. There was little difference in the infective titer of HC virus estimated between the END method performed by the established technique and the same method by the conventional technique with test tubes and swine testicle (ST) cells. Besides, there was a high correlation between the neutralizing antibody titer measured by the one technique and that measured by the other. The coefficient of correlation was r = +0.948 in this case. From the experimental results mentioned above it was concluded that the END method by the micro-technique with CPK cells was simpler than and as reliable as the same method conducted by any conventional technique, and that it was a practicable one capable of testing many samples.
采用微量板和CPK猪肾细胞株的蚀斑减少中和试验（END）法对猪霍乱（HC）病毒及其抗体进行了检测。为建立该方法的技术，对新城疫病毒（NDV）毒株类型、该病毒攻毒时间以及培养液中血清浓度等该方法的基本条件进行了研究。采用既定技术进行的END法与采用传统试管和猪睾丸（ST）细胞技术进行的END法所测得的HC病毒感染滴度差异不大。此外，两种技术所测得的中和抗体滴度之间具有高度相关性。在这种情况下，相关系数r = +0.948。根据上述实验结果得出结论，采用CPK细胞的微量技术进行的END法比任何传统技术进行的相同方法更简单且同样可靠，并且它是一种能够检测许多样本的实用方法。
