Orloski J M, Fritz P J, Liu D K
Eur J Cancer Clin Oncol. 1982 Jan;18(1):99-106. doi: 10.1016/0277-5379(82)90032-3.
The transplantable R3230AC mammary adenocarcinoma was grown in virgin, pregnant and lactating rats and in vivo rates of [3H]-thymidine incorporation into DNA and acid-soluble dTTP were compared between tumor and host mammary glands. The tumor differed from the host gland in that the rates of uptake and phosphorylation of the injected thymidine remained unchanged throughout the lactation cycle, but the dTTP pool increased greatly during pregnancy and declined during lactation. In both tumor and host gland, DNA labeling rates were higher during pregnancy than during lactation. Tumor DNA synthesis rates, in terms of incorporated dTMP, increased markedly during pregnancy and returned to pre-pregnant rates following parturition and during lactation. This pattern was similar to host mammary glands, but the change was of a greater magnitude. The data illustrate the usefulness of growing a transplantable mammary tumor in rats of varying physiological states. This way, similarities and differences between a mammary tumor and the host mammary gland regarding their responses to the hormonal milieu of pregnancy and lactation can be assessed.
将可移植的R3230AC乳腺腺癌接种于未孕、妊娠和泌乳大鼠体内，比较肿瘤和宿主乳腺中[3H] - 胸苷掺入DNA及酸溶性脱氧胸苷三磷酸（dTTP）的体内速率。肿瘤与宿主腺体的不同之处在于，注入的胸苷摄取和磷酸化速率在整个泌乳周期保持不变，但dTTP池在妊娠期间大幅增加，在泌乳期间下降。在肿瘤和宿主腺体中，妊娠期间的DNA标记率均高于泌乳期间。以掺入的脱氧胸苷一磷酸（dTMP）计算，肿瘤DNA合成速率在妊娠期间显著增加，分娩后及泌乳期间恢复到妊娠前水平。这种模式与宿主乳腺相似，但变化幅度更大。这些数据表明在不同生理状态的大鼠中培养可移植乳腺肿瘤的实用性。通过这种方式，可以评估乳腺肿瘤与宿主乳腺在对妊娠和泌乳激素环境反应方面的异同。
