Sharoni Y, Topal M C, Tuttle P R, Berger H
Thromb Haemost. 1982 Apr 30;47(2):166-72.
Of the two cell types it was possible to culture from the dissociated rat liver, hepatocytes and Kupffer cells, only the former were fibrinolytically active. Rat hepatocytes during the first 24 hr in culture secreted two plasminogen activators with molecular weights identical to those found in rat plasma, an 80,000-dalton form (PA-80) and a 45,000-dalton form (PA-45). Partially purified preparations of plasminogen activators from both sources were subjected to isoelectric focusing (IEF) to compare characteristics further. There were three distinct peaks of PA-45 in each preparation with isoelectric points of 7.1, 7.2 and 7.4; all electrophoretic forms had the same low affinity to fibrin. PA-80 from both sources displayed similar IEF profiles with forms ranging from pH values of 7 to 8, all with the same high affinity to fibrin. The major form of PA-80 in the plasma preparation had an isoelectric point of 7.9 whereas that in the hepatocyte preparation had an isoelectric point of 7.6. The isolated perfused rat liver was also shown to produce both PA-80 and PA-45 emphasizing the physiological relevance of the findings with hepatocytes. It is concluded that in the rat hepatocytes contribute to the plasma profile with regard to the plasminogen activator content.
从解离的大鼠肝脏中能够培养出两种细胞类型，即肝细胞和库普弗细胞，其中只有前者具有纤溶活性。培养的大鼠肝细胞在最初24小时内分泌两种与大鼠血浆中分子量相同的纤溶酶原激活剂，一种80000道尔顿的形式（PA - 80）和一种45000道尔顿的形式（PA - 45）。对来自这两种来源的纤溶酶原激活剂的部分纯化制剂进行等电聚焦（IEF）以进一步比较其特性。每种制剂中PA - 45有三个不同的峰，等电点分别为7.1、7.2和7.4；所有电泳形式对纤维蛋白的亲和力都很低。来自两种来源的PA - 80显示出相似的IEF图谱，pH值范围为7至8，所有形式对纤维蛋白都具有相同的高亲和力。血浆制剂中PA - 80的主要形式等电点为7.9，而肝细胞制剂中PA - 80的主要形式等电点为7.6。分离的灌注大鼠肝脏也显示能产生PA - 80和PA - 45，这强调了与肝细胞相关研究结果的生理相关性。结论是，在大鼠中，肝细胞在纤溶酶原激活剂含量方面对血浆成分有贡献。
