Chemical nature of alcoholic hyalin.

Alcoholic hyalin was prepared from postmortem livers of patients with alcoholic liver disease. Alcoholic hyalin was purified by discontinuous sucrose density gradient ultracentrifugation. The purified protein preparation contained about 12% reducing sugar, the remainder being protein. Gel filtration on Sephadex 4B column showed the presence of three peaks. Each peak material thus obtained contained acetylglucosamine, hexosamine, and deoxysugar (calculated as rhamnose). That these protein components followed by scanning the gels at 280 nm, and at 520 nm after staining the gels with periodic acid-Schiff base reagent for the presence of carbohydrate.