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重金属与核苷酸的相互作用。15. 使用拉曼差光谱法研究小牛胸腺DNA与亲电试剂硝酸甲基汞（II）、顺式二氯二氨铂（II）和反式二氯二氨铂（II）的反应。金属化后形成C-DNA的证据。

Heavy metal-nucleotide interactions. 15. Reactions of calf thymus DNA with the electrophiles methylmercury(II) nitrate, cis-dichlorodiammineplatinum(II), and trans-dichlorodiammineplatinum(II) studied using Raman difference spectroscopy. Evidence for the formation of C-DNA upon metalation.

作者信息

O'Connor T, Bina M, McMillin D R, Haley M R, Tobias R S

出版信息

Biophys Chem. 1982 Jun;15(3):223-34.

PMID:7201858
Abstract

This study details the reactions of the electrophiles CH3Hg(NO3), cis-[PtCl2(NH3)2] (cis-DDP) and trans-[PtCl2(NH3)2] (trans-DDP) with calf thymus DNA using Raman and Raman difference spectroscopy. The order of CH2Hg(II) binding to calf thymus DNA is G greater than or equal to T greater than C greater than A. The electrophilic attack of cis- and trans-DDP on calf thymus DNA produces different orders of binding cis-DDP-G greater than C approximately A much greater than T, trans-DDP-G approximately C approximately A much greater than T. The reaction of CH3Hg(II) with DNA results in a decrease in the percentage of B-form DNA, whereas the reactions of cis-and trans-DDP with DNA decrease the percentage of B-DNA and cause the formation of C-DNA structure.

摘要

本研究利用拉曼光谱和拉曼差示光谱详细阐述了亲电试剂甲基汞（硝酸酯）、顺式二氯二氨合铂（顺铂）和反式二氯二氨合铂（反铂）与小牛胸腺DNA的反应。二价甲基汞与小牛胸腺DNA的结合顺序为鸟嘌呤（G）≥胸腺嘧啶（T）＞胞嘧啶（C）＞腺嘌呤（A）。顺铂和反铂对小牛胸腺DNA的亲电攻击产生了不同的结合顺序：顺铂-G＞C≈A＞＞T，反铂-G≈C≈A＞＞T。甲基汞（II）与DNA的反应导致B型DNA百分比降低，而顺铂和反铂与DNA的反应则降低了B-DNA的百分比并导致C-DNA结构的形成。

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