可溶性乙醛脱氢酶与大豆类菌体对醛的代谢
Soluble aldehyde dehydrogenase and metabolism of aldehydes by soybean bacteroids.
作者信息
Peterson J B, LaRue T A
出版信息
J Bacteriol. 1982 Sep;151(3):1473-84. doi: 10.1128/jb.151.3.1473-1484.1982.
Abstract
A soluble aldehyde dehydrogenase (EC 1.2.1.3) was partially purified from Rhizobium japonicum bacteroids and from free-living R. japonicum 61A76. The enzyme was activated by NAD+, NADH, and dithiothreitol, and it reduced NAD(P)+. Acetaldehyde, propionaldehyde, butyraldehyde, benzaldehyde, and succinic semialdehyde were substrates. The Km for straight-chain aldehydes decreased with increasing carbon chain length. The aldehyde dehydrogenase was inhibited by 6-cyanopurine, but not by metronidazole. These compounds inhibited acetylene reduction, but not respiration, by isolated bacteroids.
摘要
从日本根瘤菌类菌体和自由生活的日本根瘤菌61A76中部分纯化出一种可溶性乙醛脱氢酶（EC 1.2.1.3）。该酶被NAD⁺、NADH和二硫苏糖醇激活，并能还原NAD(P)⁺。乙醛、丙醛、丁醛、苯甲醛和琥珀酸半醛是其底物。直链醛的米氏常数随碳链长度增加而降低。该乙醛脱氢酶受到6-氰基嘌呤的抑制，但不受甲硝唑的抑制。这些化合物抑制分离出的类菌体的乙炔还原，但不抑制其呼吸作用。
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