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[储存和短期培养对小鼠骨髓干细胞增殖的影响]

[The effect of storage and short term cultivation the proliferation of mouse bone marrow stem cells].

作者信息

Viktora L, Hermanová E

出版信息

Folia Haematol Int Mag Klin Morphol Blutforsch. 1977;104(3):388-95.

PMID:72026
Abstract

Suspension of stored and short-term cultured murine bone marrow cells was i. v. administered to lethally irradiated mice. We demonstrated that the cells still after 72 h of storing and 24 h of culture in liquid medium were capable of proliferation and on day 9 following transplantation could produce colony forming units in the spleens of lethally irradiated mice. We established the relative participation of individual types of cells in the recovery of haemopoiesis in the spleens.

摘要

将储存的和短期培养的小鼠骨髓细胞悬液经静脉注射给接受致死剂量照射的小鼠。我们证明,在液体培养基中储存72小时和培养24小时后的细胞仍具有增殖能力,并且在移植后第9天能够在接受致死剂量照射的小鼠脾脏中产生集落形成单位。我们确定了脾脏造血恢复过程中各类细胞的相对参与度。

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